FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_30.35100000038dc0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_30.35100000038dc0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802796
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG1550.019.3577024
TGGGGGG2800.015.5380135
AGGGGGG2450.015.3083875
CTACACA2451.8189894E-1212.246714
TAGGTAG2257.094059E-1112.0017755
GACCCGC900.00400998111.66612711
TGCGACG1951.5332262E-811.53824830
ACCGTGT1303.8481347E-511.5379288
GTATTAG4300.011.5133311
ATACAGG5100.011.4722856
GCTCGCG1059.7192323E-411.42804312
TACACTG3300.011.3653175
TTGGGGG2259.094947E-1011.335014
GTCCTAC950.00599224611.0542671
ACACTGT3300.010.9107056
CCTAATA5650.010.8865662
TAATACT9000.010.8349364
TAGTCAG2251.10158E-810.6661739
GGACTGC1551.9523957E-510.6467366
ACAGCGT2556.4028427E-1010.5877478
Produced by FastQC (version 0.11.2)