##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HK5NHBGXX_l01n01_ntr_3.351000000386d4.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8 Sequences flagged as poor quality 0 Sequence length 36 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.5 0.0 0.0 0.0 0.0 0.0 2 32.0 0.0 0.0 0.0 0.0 0.0 3 32.0 0.0 0.0 0.0 0.0 0.0 4 32.0 0.0 0.0 0.0 0.0 0.0 5 32.0 0.0 0.0 0.0 0.0 0.0 6 33.25 0.0 0.0 0.0 0.0 0.0 7 36.0 0.0 0.0 0.0 0.0 0.0 8 32.25 0.0 0.0 0.0 0.0 0.0 9 36.0 0.0 0.0 0.0 0.0 0.0 10 36.0 0.0 0.0 0.0 0.0 0.0 11 32.75 0.0 0.0 0.0 0.0 0.0 12 34.125 0.0 0.0 0.0 0.0 0.0 13 32.25 0.0 0.0 0.0 0.0 0.0 14 36.0 0.0 0.0 0.0 0.0 0.0 15 33.25 0.0 0.0 0.0 0.0 0.0 16 32.75 0.0 0.0 0.0 0.0 0.0 17 32.75 0.0 0.0 0.0 0.0 0.0 18 33.25 0.0 0.0 0.0 0.0 0.0 19 33.25 0.0 0.0 0.0 0.0 0.0 20 32.75 0.0 0.0 0.0 0.0 0.0 21 32.75 0.0 0.0 0.0 0.0 0.0 22 35.5 0.0 0.0 0.0 0.0 0.0 23 33.0 0.0 0.0 0.0 0.0 0.0 24 34.125 0.0 0.0 0.0 0.0 0.0 25 35.5 0.0 0.0 0.0 0.0 0.0 26 36.0 0.0 0.0 0.0 0.0 0.0 27 33.25 0.0 0.0 0.0 0.0 0.0 28 36.0 0.0 0.0 0.0 0.0 0.0 29 33.25 0.0 0.0 0.0 0.0 0.0 30 36.0 0.0 0.0 0.0 0.0 0.0 31 35.0 0.0 0.0 0.0 0.0 0.0 32 35.5 0.0 0.0 0.0 0.0 0.0 33 33.25 0.0 0.0 0.0 0.0 0.0 34 33.25 0.0 0.0 0.0 0.0 0.0 35 36.0 0.0 0.0 0.0 0.0 0.0 36 30.0 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 1.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 5.0 35 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.0 25.0 25.0 0.0 2 12.5 12.5 50.0 25.0 3 12.5 37.5 37.5 12.5 4 12.5 12.5 37.5 37.5 5 12.5 37.5 37.5 12.5 6 37.5 50.0 12.5 0.0 7 25.0 25.0 50.0 0.0 8 12.5 62.5 12.5 12.5 9 50.0 12.5 12.5 25.0 10 12.5 0.0 87.5 0.0 11 62.5 25.0 12.5 0.0 12 37.5 25.0 37.5 0.0 13 12.5 50.0 0.0 37.5 14 25.0 37.5 25.0 12.5 15 25.0 50.0 0.0 25.0 16 12.5 62.5 12.5 12.5 17 37.5 12.5 25.0 25.0 18 12.5 37.5 0.0 50.0 19 12.5 25.0 50.0 12.5 20 25.0 12.5 37.5 25.0 21 12.5 50.0 25.0 12.5 22 0.0 25.0 12.5 62.5 23 12.5 50.0 25.0 12.5 24 12.5 37.5 37.5 12.5 25 25.0 12.5 50.0 12.5 26 12.5 50.0 25.0 12.5 27 0.0 37.5 62.5 0.0 28 0.0 25.0 37.5 37.5 29 12.5 12.5 62.5 12.5 30 25.0 25.0 25.0 25.0 31 12.5 37.5 50.0 0.0 32 37.5 25.0 37.5 0.0 33 12.5 12.5 62.5 12.5 34 0.0 0.0 50.0 50.0 35 37.5 12.5 25.0 25.0 36 25.0 25.0 50.0 0.0 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.5 25 1.0 26 1.0 27 1.0 28 1.0 29 0.5 30 0.0 31 0.0 32 0.5 33 1.0 34 1.0 35 1.5 36 2.0 37 2.0 38 1.5 39 1.0 40 0.5 41 0.0 42 0.0 43 0.5 44 1.0 45 1.0 46 0.5 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.5 58 1.0 59 1.0 60 0.5 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 36 8.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 100.0 #Duplication Level Percentage of deduplicated Percentage of total 1 100.0 100.0 2 0.0 0.0 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTGTAGACTTGAGTGACCATTTCATCTTCAATACGA 1 12.5 No Hit GTACAATGATGACGCATAGTTCACGATATGTTTCTT 1 12.5 No Hit GAACAAAAGGGTAAAAGCCCCCTTGATTTTGATTTT 1 12.5 No Hit ATTGGGTAGTGGACAACCAGACAATGACACTGTTGT 1 12.5 No Hit ACTCTGTTGTGGCACCGCTAACAGTAACGGTGGCCG 1 12.5 No Hit TCCTCAGACTTTCTGATGTCAAATCTTCTAAATTGA 1 12.5 No Hit TGATTTGACTATAGATAATTGCTTTTTATCTGCTAT 1 12.5 No Hit GTTATATAGTAGAAAGGATGAAGAAAATTTATTCCG 1 12.5 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.0 22 0.0 0.0 0.0 23 0.0 0.0 0.0 24 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE