FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_29.35100000038d8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_29.35100000038d8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711473
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2750.018.5499365
TAGGGGG952.6306236E-514.2139594
TAGGTAG1803.274181E-1013.3365555
TGCGACG2153.274181E-1112.55933930
TACAGGC3150.012.3792417
TTGGGGG2257.094059E-1112.00294
GTGCGAC2257.094059E-1112.00114529
GTATTAG4400.011.5937091
ACCTAGT1459.31266E-611.382061
GTAGGAC1202.3401318E-411.2527183
GGTAGTC2351.879016E-910.8495647
AGGGGGG1408.1585116E-510.7168745
CACGCAG2556.4210326E-1010.58677116
CAACACG2701.546141E-1010.55409613
TAATACT9100.010.5519994
ACTGTGC2002.6930502E-710.49854858
TTAAGAC2301.5441401E-810.4373043
GGTGCGA2608.949428E-1010.38560728
TATACGG4200.010.3596455
TAGACAG2905.0931703E-1110.3473275
Produced by FastQC (version 0.11.2)