FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_28.35100000038d4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_28.35100000038d4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456008
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGG200.005147271330.00051525
TGGGGGG2200.019.7737465
TAGGGGG859.2021455E-615.883174
AGGGGGG1658.185452E-1114.5462035
CCTAGCG750.001017778314.00072130
ACCGTGT800.001657419513.1243258
TTAGGGG1302.7582264E-612.6929623
TTGGGGG2051.9281288E-1012.4396664
GTATTAT7900.012.1525251
GTCCGAA1252.5761026E-511.99938111
GACGATC1151.5797102E-411.73852522
ACCGGAG1059.7204995E-411.42758924
TAGGTAG2002.2244421E-811.250585
ATACGGT3900.011.1544216
ACACCGT2207.7398E-910.9096536
TATACGG4150.010.8439325
GTATAGA4300.010.814511
GTATTAG3650.010.6854821
GTGCGAC1150.002074431910.43532129
TACGGTG3550.010.1399757
Produced by FastQC (version 0.11.2)