FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_27.35100000038ceb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_27.35100000038ceb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824549
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2050.021.220875
AGGGGGG2850.017.3695325
TTGGGGG1750.016.2867574
CCGTGAC750.001018278114.0001289
TAGGGGG2101.8189894E-1213.5722974
GCTAGCC902.6524608E-413.3338213
CTGCGCA954.1891908E-412.6316959
CTAGGAC1354.209871E-612.2226693
CCGGATC1252.5746189E-512.00043825
CAGTCCA1754.236972E-812.000119
TATACGG5200.011.53925
CCTATAC3400.011.4710073
GTATTAA5900.011.4410961
CTAATAC5450.011.2848173
TAGACGG1755.473903E-711.143575
TATACTG6950.011.0078995
GGGAGTC1100.001433600810.9088917
TCTATAC6100.010.8200683
CCGAGCC1253.4239978E-410.79980314
GACTAGG1000.00877980610.5000958
Produced by FastQC (version 0.11.2)