FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_26.35100000038ca8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_26.35100000038ca8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241160
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC801.1061275E-820.62386313
TGGGGGG1950.018.4634975
CCGAGCC908.588686E-716.66574914
TAGTCAG2150.016.0456289
TAGGTAG2100.015.7159545
GAGACCG901.5813717E-515.00038224
GGTAGTC2200.014.9991747
CTCCGAG1002.5753761E-614.99917312
CCATGCG1402.99151E-813.9278059
TAGGGGG650.006381939613.8476234
CGCGTAT800.001657224613.12427610
GTAGTCA2750.013.0901888
TTGGGGG2300.013.0448624
GTATTAG3850.012.8585071
CTAATAC3900.012.6936553
GTATTAA3750.012.4013171
CCACGAG1406.313234E-611.786015520
ATACGGT3250.011.5396866
CGTACTA1303.8446455E-511.53829229
TTAGGTA3000.011.5012214
Produced by FastQC (version 0.11.2)