FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_25.35100000038c64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_25.35100000038c64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591735
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG755.2314135E-922.0032845
ACCTAAT1700.016.7672081
CCCCAGA550.002097926816.3660812
AGGGGGG1104.1826934E-715.0022415
CTAATAC1900.015.0022393
CCAGCGC600.003753657714.9984368
CCTAATA2350.013.4062582
TAATACT2900.012.9329654
CTATGCA2102.1827873E-1112.85580359
CCTATAC954.1768217E-412.6334653
TATACGG954.1768217E-412.6334655
TACAGCG954.1857653E-412.6302627
CTTAAGA1201.6873846E-512.5018652
AATACTA2650.012.4546895
CTCTTAT1456.8643203E-712.4156471
GAAGTGT850.002606756512.3547856
CTCCGTC850.00261006412.35269716
TATACAG1951.1550583E-912.309535
GGCGTCC1101.0422619E-412.27144921
AGTATGC1006.4464676E-411.9987498
Produced by FastQC (version 0.11.2)