FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_24.35100000038c14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_24.35100000038c14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643145
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGA12790.19886650755272917No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAG6530.10153231386390317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCAG651.6444013E-518.4611079
TAGAGGA2350.017.24034330
TAGGTAG703.1075237E-517.1437915
TACAGTC550.002099525416.3645275
TAACTGT550.002099525416.3645274
TCTATCC1401.07320375E-1016.0710543
CCCTGCC755.6158067E-515.9996269
CTCTTAT2400.015.6246361
TATCCTT1451.8189894E-1015.51808555
GTAGAGG2600.015.00548429
TACCCTA706.0292415E-415.0008175
GCACGAC809.749924E-514.99848413
CACGACG901.5809113E-514.99848414
TTATCCT1554.965841E-1014.516924
ATACTAT1554.965841E-1014.516926
GTATTAG1156.9317684E-714.3474921
GCGTCCT1054.267182E-614.28426922
AAGGGTG952.6302267E-514.21135
GGTAGTC851.6320021E-414.1173177
ATGTGTG750.001017268913.9996737
Produced by FastQC (version 0.11.2)