FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_23.35100000038bdf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_23.35100000038bdf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1619387
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG1850.015.4102285
TAGGGGG1709.094947E-1215.0046964
TGCGCGC901.58367E-514.99867220
CTATGCA4050.014.4431659
GCGCGCC851.6353253E-414.11639721
CGCGCCT851.6353253E-414.11639722
TAGGTAG2450.013.4736045
CTAATAC6250.013.4437913
GACTGCT1601.2141754E-813.1238377
TCACACA4250.012.706326530
CCTACAC1302.7516508E-612.69588853
GGTAGTC2153.274181E-1112.5570287
GTATTAG4000.012.3784911
TTGCGCG850.00261539912.35146719
ACTATGC5500.012.2716418
ACTGCGC1101.0442697E-412.2716418
ATCACAC4500.012.00042129
GACGCGC900.00401094611.6656338
TTAGGGG1703.7509562E-711.4738243
AGGGGGG2751.8189894E-1211.4581315
Produced by FastQC (version 0.11.2)