FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_22.35100000038b9c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_22.35100000038b9c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765163
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGCC500.001110411117.99843214
ATAGGAC1051.3224053E-817.1469673
TAGGACC703.1038326E-517.1469654
TAGGGGG550.00209741816.3675584
GTGTAGG653.428472E-416.1577171
GTATTAG1401.07320375E-1016.0752811
ACTGCGA706.037582E-414.99869258
CCAGCGC600.003754570114.99869258
TCTCCGA706.037582E-414.998692511
CTAGACT750.001015557914.0033554
CTAAGCA750.001015557914.0033554
CACTGGG750.001018069913.998787
CGAGGCT650.006378871413.84766229
ACAGAGC902.6520868E-413.3321718
CGTGAGC800.001656613213.12385712
GACCGAG800.001656613213.12385726
CTAATAC2203.6379788E-1212.957653
TGGGGGG850.002606194512.3559025
GGTCATA1006.4316555E-412.0028761
CCTGTAC900.003997826511.6694623
Produced by FastQC (version 0.11.2)