FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_21.35100000038b59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_21.35100000038b59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128996
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCT2270.17597444882011845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT254.1914385E-430.01746230
CGATCTG200.00512565530.0058223
GCTACGC359.869166E-525.72925429
GAGTAGC300.001222037224.9951536
TCTCCTC300.001222037224.9951535
CTCCGAG350.002995235421.42441712
CTATACT350.002995235421.4244174
CCGAGCC350.002995235421.42441714
TGTTACG350.002995235421.42441718
TCTCCGA350.002995235421.42441711
AGTAGCC350.002995235421.4244177
ATACACA504.5688925E-520.995934
ATTATAC455.4376037E-419.9961223
CGCTACG400.006458163718.76091428
GAGACGC400.006472658418.75363724
CGAGACG400.006472658418.75363723
ACGAGAC400.006472658418.75363722
TCCGAGC400.006487179518.74636713
AGCCCAC400.006487179518.74636717
TATTAGG400.006487179518.7463672
Produced by FastQC (version 0.11.2)