FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_20.35100000038b15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_20.35100000038b15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347284
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG501.6906906E-624.0020755
TAGTCAG601.8617703E-417.4989919
CTATGCA908.5452484E-716.6657079
GTTATAA550.0020964616.3650511
TATCCCG550.0020964616.3650515
ACCTAAT859.158801E-615.8837261
GTATTAG1052.4609653E-715.7156441
GTATAAC809.7138414E-515.0012971
TTAGGTA706.0178863E-415.0012964
ATCACAC706.0178863E-415.00129629
GAAGTGT600.003744744715.0012966
AATACTA1401.8317223E-915.0012965
ACCCGAA600.00374833514.99913617
CGCCGCG600.00374833514.99913624
CCTAATA1054.2453103E-614.2869492
TCACACA851.627073E-414.11886830
GTATAAT851.627073E-414.1188681
TCAGATG851.6290652E-414.11683512
ATTTAAT750.001014771414.001211
ATTAGGT750.001014771414.001213
Produced by FastQC (version 0.11.2)