FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_19.35100000038ad0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_19.35100000038ad0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717682
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAGTC2850.017.8945277
GTATTAG4650.017.4201661
CCTAGCG1800.015.83407230
TAGGTAG3900.015.0011365
TAGGGGG1301.1268639E-815.0011364
TGGGGGG2300.015.0011355
TAGTCAG3300.014.5452859
CTAATAC6550.014.4281533
TTGGGGG1608.039933E-1014.0635654
CTATGCA4650.013.8708069
TAATACT8500.013.7657494
CTAGGCA2200.013.63620510
ATCACAC4250.013.41239129
ACCTAAT5400.013.3339541
CCTAATA6200.013.3070732
CCCCTAC1251.7769507E-613.2006151
TTAGGTA4550.013.1878124
GCGCACT1601.2134478E-813.12446511
TCACACA4200.012.85774230
CCGAGCC1302.7619008E-612.6917915
Produced by FastQC (version 0.11.2)