FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_18.35100000038a9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_18.35100000038a9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047789
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG2100.017.8589614
TAGGTAG3150.016.1921235
CGTCCGG706.032818E-415.00225930
GCCGCGT2000.015.0011625
TGGGGGG3500.014.572915
CGCCGCG2100.014.28507524
TTGGGGG2500.013.8014054
AGGGGGG3850.013.2481015
CGGGTCC1151.0930682E-513.04448725
GTATTAG6300.012.1437951
CCTAATA6450.012.0939592
GGTAGTC3350.012.088427
CTAATAC6450.011.86138253
CGATCAC2206.2937033E-1011.5903924
GTATAGA5050.011.5850541
CCGAGCC1859.17571E-811.35056614
GGCGTCC2659.094947E-1211.32024921
GCGTCCT3050.011.31096922
TAGTCAG3850.011.2976449
TTAGGTA4350.011.0356054
Produced by FastQC (version 0.11.2)