FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_17.35100000038a5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_17.35100000038a5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535258
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG5500.018.2745531
GGTAGTC2850.017.3673297
TAGGGGG1152.220986E-916.9582164
TAGGTAG3350.015.6732085
TAGTCAG3100.014.9990549
TGGGGGG1251.1321754E-714.4014395
CTAATAC5350.014.0200923
ATCACAC3650.013.97399929
CTATGCA4000.013.8741269
TATTAGG5300.013.5862632
TTAGCCG1302.7620463E-612.69150914
GCCGCAG1302.7620463E-612.69150917
GGTGATC1354.213327E-612.2214538
ACTATGC5050.012.1774518
TCACACA4200.012.14407130
CCTAGCG1252.5723253E-512.00119930
TCCGAGC1006.454559E-411.99924513
AGGTAGT4200.011.7868926
AGGGGGG2206.2755134E-1011.5920675
TTAGGTA5700.011.5801044
Produced by FastQC (version 0.11.2)