FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_16.35100000038a09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_16.35100000038a09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1708965
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2600.019.6183325
GGTAGTC2300.016.95517
GTCCTAA1655.456968E-1215.4568681
TAGGTAG2850.015.2654515
ACCCCCC600.003757013714.99874119
TAGGGGT750.001017146314.0021034
TAGTCAG2800.013.9274039
TTGGGGG2700.013.8909764
CGGTCGG650.006391276613.84499213
CGGGTCC1056.751403E-512.85756925
TAGGGGG1651.8613719E-812.7291864
TCGGGGG954.1922377E-412.63051916
GTCGGGG850.002614804812.35190515
AGGGGGG2451.8189894E-1212.24673755
GTATTAG4700.012.1294821
GGGGCTC1151.5804941E-411.73814610
TATTAGG4950.011.2138052
ACTATGC5550.011.08015258
TTAGGTA4650.010.9693894
TGACCTG1653.267689E-610.910735
Produced by FastQC (version 0.11.2)