FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_15.351000000389d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_15.351000000389d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736492
Sequences flagged as poor quality0
Sequence length36
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG2850.015.7907175
GCACCGC600.003752983415.0011826
GGCGCCG800.001656378913.12565525
CACCGCG800.001656378913.12565526
GTATTAG4700.013.0861371
GTGCCCC954.192657E-412.630399
TAGTCAG2800.012.320279
GGTAGTC2850.012.1041257
TAGGTAG3550.011.8319185
GCACTGC900.004006136711.6675856
CAGCTCG900.004011311611.6655689
ACCGTGC900.004011311611.6655688
TTGGGGG2206.2755134E-1011.5918214
ATACGGT5100.011.4714916
GTAGTCA3400.011.4695088
ACGGTGT4350.011.378248
TACGGTG4050.011.1100667
GCTAGCC1100.001432380510.9099493
GAGAGTC1807.888284E-710.8323147
GTCCTAA2251.0992153E-810.6675061
Produced by FastQC (version 0.11.2)