FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_14.3510000003899e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_14.3510000003899e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584680
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG1750.015.4309895
TAGGGGG1251.7745042E-613.2020694
CTAGGAG1507.3718184E-813.0020373
AGGGGGG1855.0567905E-1012.9750075
CACGCAG1907.748895E-1012.6307716
CTAATAC5000.012.6019753
CCGGTCA1101.0440045E-412.271949
TCTAGGA1901.0408257E-811.8439632
TCTAAGC1355.6413443E-511.1128533
TATACTG6100.011.0673075
CCTATAG1653.266945E-610.9108013
TTGGGGG1851.1185257E-610.5421934
GGTGCGA1851.1198881E-610.54119528
ACCCTCG1000.008783719510.4993277
GTCTAGG1304.909426E-410.3862431
ACTATGC4350.010.3441648
CTAAGCA1756.6012944E-610.2873264
CTATGCA3650.010.2733149
TTATACT6850.010.0745724
GCCTAGA1200.002951077210.0015681
Produced by FastQC (version 0.11.2)