FastQCFastQC Report
Tue 3 Nov 2015
HK5NHBGXX_l01n01_ntr_13.3510000003895b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5NHBGXX_l01n01_ntr_13.3510000003895b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893545
Sequences flagged as poor quality0
Sequence length36
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG901.583205E-514.999429
TAGGGGG1351.8535502E-814.4454114
TAGGTAG3000.013.000875
AGGGGGG2700.012.2230415
GTATTAG5250.011.715071
TGGGGGG2052.561137E-911.7081015
TAGTCAG2850.011.5784999
TAGGACC2104.564572E-810.7150024
GTGCGAC1408.176237E-510.7147229
ACACAGC2701.546141E-1010.5562626
GTATAGG3253.6379788E-1210.1545251
GGTCTAT2402.9991497E-810.0006691
GGTAGTC3152.3646862E-119.9993497
GTATAAG5600.09.9113781
CCTACAC1705.4022523E-59.7065323
ACCGTGT1409.7189273E-49.6424848
TCGCACC1250.00414367359.59962821
TATTAGG6650.09.4743182
GTCTACA3655.456968E-129.4526881
ATAGGGG1952.3866149E-59.2313873
Produced by FastQC (version 0.11.2)