FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02n02_atac_root_aug_rep3_3.352000000ff580.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02n02_atac_root_aug_rep3_3.352000000ff580.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27391041
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT147200.024.11956245
CTGACGC263400.019.2876145
TCTGACG356400.015.04376545
ATCTGAC456900.011.80367144
TATACAC1668250.011.22102245
ATACACA1269950.010.32531145
CATCTGA533450.010.09693443
CTATACT51950.010.0522064
GTCTTAG25000.09.9042761
GTGTAGA49800.09.7632511
TTATACA2133550.09.75564845
GTATAGG48650.09.577621
TAGACCG30350.09.2706355
GTATTGG61700.09.26656151
GTCTAGG30500.09.2252941
TATACTG58250.09.1968525
TAGGACT38850.09.1542984
GGACCGT49450.09.0541126
GTCCTAG32800.08.9901621
ACTGTGC51700.08.9647018
Produced by FastQC (version 0.11.2)