FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02n02_atac_root_aug_rep3_2.352000000ff566.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02n02_atac_root_aug_rep3_2.352000000ff566.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23890758
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT119000.022.78282445
CTGACGC211650.018.2523845
TCTGACG274650.014.46700245
TATACAC1274850.011.7115645
ATCTGAC349300.011.44606744
ATACACA956400.010.56738845
GTGTTAG34000.010.1957741
TTATACA1650250.010.1531145
GTATAGG38450.010.0110171
CATCTGA405200.09.84463243
GTATTAG34550.09.70770551
GTCCTAG30450.09.6102451
TAGACAG28700.09.4900645
GTGTAGG42250.09.4835661
ACTGTGC46000.09.3907828
GTCTTAG19000.09.3594841
GTCTAGG29050.09.1435381
GTATAAG37900.09.0872111
CTATACT47200.09.0610434
GTGTAGA42550.09.0463821
Produced by FastQC (version 0.11.2)