FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02n02_atac_root_aug_rep3_1.352000000ff54d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02n02_atac_root_aug_rep3_1.352000000ff54d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23595842
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT102900.022.040245
CTGACGC172950.016.32651545
TCTGACG217750.013.56678445
TATACAC1125050.012.94906145
ATACACA812400.011.70942145
TTATACA1487300.010.96906745
ATCTGAC279000.010.66099244
TAGACAG27200.010.013415
GTATAGG44150.09.8402041
GTATTGG55650.09.6269621
GTCCTAG30150.09.5565271
GGACCGT43100.09.5006326
TACACCG42600.09.4053715
ACTGTGC44800.09.391228
GTCTAGA30000.09.37920951
GTGTTAG36750.09.3715521
CATCTGA318850.09.32130243
CTAAGAC21850.09.2716413
CTATACT48900.09.16034
CTTATAC1846500.09.09357145
Produced by FastQC (version 0.11.2)