FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02n02_atac_root_aug_rep2_1.352000000ff523.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02n02_atac_root_aug_rep2_1.352000000ff523.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25846712
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT118950.023.776145
CTGACGC228300.019.31612445
TCTGACG301900.015.05421445
ATCTGAC392350.011.62958144
TATACAC1342950.011.10600845
GTCTTAG24700.010.7537081
GTGTAGG48350.010.5682371
CATCTGA454550.09.99839443
ATACACA1025400.09.90901345
GTCCTAG35400.09.7924341
TTATACA1720100.09.74871245
GTGTTAG43000.09.5797851
GTATAGG44900.09.5253381
CTACACT46050.09.4827514
GGACCGT39400.09.4220766
ACCGTGC72400.09.3537728
GTATTAC38150.09.2045641
GTATTGG62350.09.1700231
ACCGTCC65700.09.1433388
CCTACAC50700.09.1013993
Produced by FastQC (version 0.11.2)