FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02n02_atac_root_aug_rep1.352000000ff509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02n02_atac_root_aug_rep1.352000000ff509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43450501
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT316850.021.81417845
CTGACGC537100.017.37620545
TCTGACG633850.015.405457545
ATCTGAC799800.012.23149344
TATACAC2857450.011.71171445
ATACACA2125650.011.05786245
CATCTGA899250.010.87100543
GTGTAGG66650.010.6384831
GTATAGG70450.010.4480691
TATACCG62350.010.3249015
TACACCG77800.010.0682965
TATACTG55850.010.0353495
GTCTTAG27000.010.0043011
TTATACA3673300.09.99375345
GTGTAGA71200.09.8954071
ACATCTG1031550.09.62070942
GTATTGG80700.09.5952041
GTATAGA65300.09.5140451
TAGACTG38250.09.3566845
GTCTAGG38900.09.3163451
Produced by FastQC (version 0.11.2)