FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02n01_atac_root_aug_rep3_3.351000000ff583.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02n01_atac_root_aug_rep3_3.351000000ff583.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27391041
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC146800.024.09265345
CTCCGAG251550.020.24036645
TCTCCGA382000.014.1647845
ATCTCCG477750.011.35421344
TATACAC1678150.011.20680145
GTCTTAG23000.010.4737431
ATACACA1275650.010.35120145
TTATACA2143200.09.74714345
GTATTAG43500.09.5747721
GTATAGG44200.09.47407151
TAGACTG32400.09.3775215
GACTGTG35300.09.3691627
GTATAAG43850.09.3443211
GTGTAGG49300.09.2703281
CTATACT53250.09.1292134
ACATCTC604750.08.93996942
GGACCGT49100.08.93681056
GTCCTAG32300.08.921821
TATACCG44150.08.9208585
TACACCG53250.08.917895
Produced by FastQC (version 0.11.2)