FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02n01_atac_root_aug_rep3_2.351000000ff569.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02n01_atac_root_aug_rep3_2.351000000ff569.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23890758
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC113650.023.95371845
CTCCGAG193000.020.01566345
TCTCCGA293000.013.62974445
TATACAC1279800.011.69762445
ATCTCCG368300.010.855412544
ATACACA958150.010.58305545
GTGTAGG40150.010.2616051
GTGTAAG29000.010.2476831
TTATACA1657850.010.16874845
GTCCTAG29650.010.0230281
TATACTG46750.09.6282665
AGACCGT33600.09.375966
TAGACTG29450.09.2469775
GTATTAG34400.09.0317191
GTATAGG41100.08.9836281
CTATACT47300.08.9453324
GTGCTAG39300.08.9367951
ACCGTCC62400.08.869698
GTATTGG53600.08.82072451
ACATCTC456900.08.80441842
Produced by FastQC (version 0.11.2)