FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02n01_atac_root_aug_rep3_1.351000000ff540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02n01_atac_root_aug_rep3_1.351000000ff540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23595842
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC104900.021.5549745
CTCCGAG161150.017.45165445
TATACAC1125350.012.95920345
TCTCCGA237900.012.57807245
ATACACA817150.011.70156745
TTATACA1494350.010.99677545
TATACTG52550.010.4071375
GTATAGA45500.010.3908721
ATCTCCG289800.010.32560844
GTCCTAG29450.010.1674121
GTGTAGG43350.09.9194681
GGACCGT39750.09.84997756
GTATAAG39450.09.644591
GTATAGG40000.09.5119781
GTGTTAG35550.09.4993911
TAGACTG28300.09.463645
TATACCG33850.09.3747175
TACACCG45600.09.1800665
CTATACT50300.09.1724124
CTTATAC1851500.09.11490845
Produced by FastQC (version 0.11.2)