FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02n01_atac_root_aug_rep2_1.351000000ff526.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02n01_atac_root_aug_rep2_1.351000000ff526.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25846712
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC116850.024.14479445
CTCCGAG212400.020.7931645
TCTCCGA319200.014.322391545
ATCTCCG398050.011.53624144
TATACAC1345200.011.13220545
TAGACAG33600.010.6501235
GTCTTAG24950.010.5581471
GTATAGG47250.010.1019791
ATACACA1030900.09.92782545
TTATACA1722400.09.7549545
GTATAGA48450.09.6194221
TATACTG56000.09.5650155
TAGACTG31500.09.4310515
GTGTAAG32500.09.21384051
ACATCTC507100.09.0375842
GTATTGG58300.09.0369041
GTCTAGA36900.09.0304161
TACACCG48700.09.011615
TATACAG51600.08.8976885
CATCTCC525250.08.85378843
Produced by FastQC (version 0.11.2)