FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l02n01_atac_root_aug_rep1.351000000ff50c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l02n01_atac_root_aug_rep1.351000000ff50c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43450501
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC243800.027.98020745
CTCCGAG432300.021.46292345
TCTCCGA664800.014.80616245
ATCTCCG825200.011.9310244
TATACAC2862050.011.73491345
ATACACA2133400.011.06685545
TATACCG59350.010.8831375
TATACTG57950.010.1751495
TTATACA3684450.010.00955445
CATCTCC998250.09.91901443
GTGTAGG66800.09.9088051
ACATCTC1003200.09.80054842
GTATAGG75000.09.5759061
ACTGTGC57250.09.5103138
GTATAGA66200.09.488481
CTATACT61200.09.4141584
TACACTG50850.09.38291555
GTGTTAG38500.09.356421
TACACCG77150.09.3348435
GTGTAAG35400.09.2217881
Produced by FastQC (version 0.11.2)