FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01n02_atac_root_aug_rep3_3.352000000ff59d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01n02_atac_root_aug_rep3_3.352000000ff59d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26724605
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT142150.023.9616645
CTGACGC263500.019.44961545
TCTGACG351050.015.12449745
ATCTGAC445050.011.97551444
TATACAC1648500.011.38054245
ATACACA1246650.010.42186345
CATCTGA518050.010.28800943
GTGTAGG47950.010.144311
TACACCG48000.09.8982395
GTCTTAG21850.09.7910571
TTATACA2099550.09.58714145
TAGACAG30650.09.4771115
TATACTG55600.09.3552185
GTATAGG44850.09.2889521
CTATACT53100.09.2868224
ACATCTG602700.09.11535142
GGACCGT47550.09.0860426
GTGTAAG34550.09.0599161
GTCTAGG29450.09.0230661
GTGCTAG43550.08.997431
Produced by FastQC (version 0.11.2)