FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01n02_atac_root_aug_rep3_2.352000000ff573.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01n02_atac_root_aug_rep3_2.352000000ff573.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23310831
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT114450.022.92014945
CTGACGC209500.018.77120245
TCTGACG278150.014.88241545
ATCTGAC357550.011.62786244
TATACAC1286550.011.54996945
ATACACA966250.010.48448645
TTATACA1656200.010.03572645
CATCTGA414950.010.00853643
GTCCTAG30250.09.7520781
GTATAGG39850.09.5501381
GTGTAAG27850.09.4604531
GTATAGA38300.09.2310711
AGACCGT34100.09.1725316
TAGGACT30550.09.0659344
TATACTG45600.08.9378175
GTGTAGG42650.08.9231651
ACATCTG477750.08.91409242
GTATAAG36450.08.896441
CCTACAC39950.08.8493963
TACACCG46900.08.8341075
Produced by FastQC (version 0.11.2)