FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01n02_atac_root_aug_rep3_1.352000000ff55a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01n02_atac_root_aug_rep3_1.352000000ff55a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23037944
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT102250.021.29835145
CTGACGC169450.016.42336345
TCTGACG211350.013.57193945
TATACAC1109750.012.57304945
ATACACA817100.011.79803245
TTATACA1470900.010.97266645
ATCTGAC274200.010.54313344
TATACTG51900.010.2393595
GTATTGG49400.09.9834181
GTATAGG41850.09.578261
TAGACAG27750.09.5751845
GTCCTAG29200.09.4860281
TACACCG43800.09.4595795
CTATACT50150.09.2945534
GTATTAG38450.09.195291
CTTATAC1829150.09.18766745
TAGACCG25600.09.14790255
TACACAG32600.09.1176825
GTATAGA41250.09.1170561
TACACTG42750.09.1125135
Produced by FastQC (version 0.11.2)