FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01n02_atac_root_aug_rep2_1.352000000ff530.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01n02_atac_root_aug_rep2_1.352000000ff530.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25239059
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT118150.023.5354445
CTGACGC220650.019.00551245
TCTGACG296700.015.43823945
ATCTGAC385100.011.93527444
GTGTAGG48950.011.3168691
TATACAC1347800.011.13366145
CATCTGA450050.010.23280443
ATACACA1030100.010.07055345
GGACCGT40600.09.9209696
TTATACA1730150.09.87991245
GTATAGG45100.09.6865491
TACCCCG27700.09.6733445
CTAGGAC33750.09.4740393
GTCCTAG34050.09.2588021
GTGTAAG33000.09.1439691
GTATAAG42450.09.1241961
GTGTAGA48750.09.0998751
ACATCTG519250.09.09854142
CTACACT41450.08.9090144
GTCTAGA34800.08.8651311
Produced by FastQC (version 0.11.2)