FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01n02_atac_root_aug_rep1.352000000ff516.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01n02_atac_root_aug_rep1.352000000ff516.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42606396
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT318000.021.83973545
CTGACGC538400.017.00280245
TCTGACG635100.015.31372845
ATCTGAC795250.012.26374944
TATACAC2833300.011.81698245
ATACACA2103850.011.06143845
CATCTGA894000.010.91666243
TACACCG75800.010.6641465
GTGTAGG65900.010.3195351
TATACCG60350.010.2975215
TTATACA3655300.010.06496445
ACTGTGC56700.09.9996768
GTATAGG70000.09.940291
TATACTG56200.09.8159115
GTCCTAT58600.09.6452771
ACATCTG1030150.09.62220942
GTATAGA63150.09.5921861
GTGTAGA70900.09.5282611
ACCGTGC162750.09.3591438
GTGTAAG32050.09.2743721
Produced by FastQC (version 0.11.2)