FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01n01_atac_root_aug_rep3_3.351000000ff590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01n01_atac_root_aug_rep3_3.351000000ff590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26724605
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC136550.024.66522245
CTCCGAG245750.020.73622345
TCTCCGA376550.014.21434145
ATCTCCG470150.011.43711444
TATACAC1653550.011.35167445
ATACACA1252750.010.38229845
GTGTAGA47800.09.98440551
GTATAGG45550.09.7856821
TTATACA2103450.09.57829945
ACCGTCC68800.09.45072658
GTCCTGG56900.09.3767061
GTGTAGG46200.09.3069121
AGACCGT40300.09.2693276
ACATCTC584250.09.18812142
GTATAGA48350.09.125861
TACCCCG31650.09.1012135
CATCTCC598300.09.08140743
GTATAAG43650.09.0770061
CTACACT43300.09.04336554
GTATTGG61050.08.9974281
Produced by FastQC (version 0.11.2)