FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01n01_atac_root_aug_rep3_2.351000000ff576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01n01_atac_root_aug_rep3_2.351000000ff576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23310831
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC107150.024.2517845
CTCCGAG187150.020.881645
TCTCCGA293550.014.24024445
TATACAC1290150.011.52347945
ATCTCCG369050.011.40623644
GTGTAGG44650.010.4874881
ATACACA975650.010.44388545
GTATAGG41000.010.0483981
TTATACA1658500.010.03310245
TACACTG41300.09.6445775
TACACCG45050.09.6410095
GTGTAAG27350.09.6307061
GTATAGA39150.09.3731481
ACCGTCC64900.09.3599758
TATACTG46500.09.3403755
GTATTAG34000.09.3361931
GTGTTAG32150.09.2432041
GGACCGT40450.09.179286
GTATTGG52700.09.1418221
ACATCTC461450.09.10276742
Produced by FastQC (version 0.11.2)