FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01n01_atac_root_aug_rep3_1.351000000ff55d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01n01_atac_root_aug_rep3_1.351000000ff55d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23037944
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC101150.021.46426845
CTCCGAG156100.017.5116945
TCTCCGA226400.012.83928245
TATACAC1109050.012.57144145
ATACACA815350.011.85151345
TTATACA1472550.010.96394945
GTGTAGG45200.010.4592261
ACTGTGC42900.010.2791068
ATCTCCG289050.010.0797944
GTCCTAG30150.09.9307721
GGACCGT37600.09.7553556
ACTGTCC34600.09.6887278
TATACTG50800.09.5243225
GTATAAG37500.09.2450261
CTTATAC1830750.09.1972845
TATACCG35550.09.1155365
TACACTG41900.09.0767845
CTACACT36400.09.0264784
TACACAT597650.08.97834245
ACCGTCC62400.08.9417738
Produced by FastQC (version 0.11.2)