FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01n01_atac_root_aug_rep2_1.351000000ff533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01n01_atac_root_aug_rep2_1.351000000ff533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25239059
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC115450.024.1257345
CTCCGAG202700.020.51171745
TCTCCGA313850.014.57367145
ATCTCCG394100.011.63460244
TATACAC1346250.011.14355345
GTGTAAG32850.010.3484231
ATACACA1029000.010.07950845
TAGACTG32950.010.0397985
TTATACA1728100.09.89981345
TATACTG56200.09.8505815
TACACCG44900.09.3224415
TATCCCG34600.09.1707755
GTACAGA51500.09.1363281
GTGTTAG41900.09.1341361
GTGTAGG46250.09.1025371
ACATCTC505500.09.09732142
GTATAGG44600.09.0859491
GTATTAG42300.08.8880961
GTGTAGA52850.08.8603521
CATCTCC527400.08.85606943
Produced by FastQC (version 0.11.2)