FastQCFastQC Report
Thu 6 Sep 2018
HK5MMBCX2_l01n01_atac_root_aug_rep1.351000000ff519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5MMBCX2_l01n01_atac_root_aug_rep1.351000000ff519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42606396
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC242600.028.49874545
CTCCGAG426750.021.22004745
TCTCCGA656700.014.83799245
ATCTCCG813700.011.97506544
TATACAC2833250.011.81524845
ATACACA2107450.011.06964245
TATACCG59300.010.5499315
TATACTG53400.010.3670065
GTGTAGG64950.010.2596881
TACACCG73650.010.1138095
TTATACA3651450.010.0858145
TACACTG50550.010.0611315
CATCTCC984350.09.95388543
ACATCTC994450.09.82790342
GTATAAG47250.09.8149261
GTATAGA62250.09.6197531
CTACACT46100.09.5191764
GTGTAAG30600.09.4905191
GTCCTAG36800.09.4209461
GTCCTAC48200.09.3412421
Produced by FastQC (version 0.11.2)