FastQCFastQC Report
Thu 13 Feb 2020
HK5KKDRXX_l02.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5KKDRXX_l02.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494700750
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG10332150.054.582
AAGCAGT11063600.051.1156271
AGTGGTA11314450.049.6906285
GCAGTGG11359650.049.6223953
CAGTGGT12954350.043.4834864
GTGGTAT13075300.042.9802636
GGTATCA13189300.042.7003758
TGGTATC13935550.040.4020427
GTATCAA14681550.038.4294629
ACGCAGA12322350.026.36137214-15
CAACGCA12518250.026.06534812-13
ATCAACG13165750.024.86067610-11
AGTACAT13104100.024.51332520-21
GCAGAGT13244300.024.48741516-17
AGAGTAC13251900.024.45367618-19
TACATGG13291300.024.121722-23
CATGGGG9467500.024.02325824-25
GTACATG12279500.022.70434422-23
TCAACGC13035200.021.7724512-13
AACGCAG13200150.021.53704314-15
Produced by FastQC (version 0.11.5)