FastQCFastQC Report
Thu 13 Feb 2020
HK5KKDRXX_l02.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5KKDRXX_l02.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494700750
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGGTGT6559939313.260419152386568No Hit
ATAGGCCA6294067512.722979498211798No Hit
CGAACCTG6236104812.605812301679348No Hit
GAGACTAT6178548812.489467218313294No Hit
ATCTTACC5856026911.837513688831077No Hit
CGCTTAGC5486859211.091269216794194No Hit
GCGCAGAA5069283710.24717205300376No Hit
TCTCAGTG4967854210.042140021012704No Hit
GGGGGGGG96129991.9431947495531388No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)