FastQCFastQC Report
Thu 13 Feb 2020
HK5KKDRXX_l02.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5KKDRXX_l02.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494700750
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCC1402600.017.1567521
GCCCGAA961250.016.7096441
CCGGACA954300.016.362041
CTGGCAG1119900.016.2840291
TGCTTGC1420750.016.236651
ACGCACG1120900.016.1968561
AGATGAC971200.015.9920618
CTGGACG747100.015.8774221
CCATGAC1039500.015.7551097
AGACGTC955350.015.6920598
AAACGCT1248500.015.6211571
ACGCGAG718050.015.5333223
CTGTGGG1006250.015.5129371
GGGATCC1107400.015.3370591
GAAGCCC980700.015.25285151
GGTTAAC1321450.015.2351021
GCGTGGT849800.015.2297643
CAGGGGT899950.015.1951063
GACACGC1283200.015.1687191
AACGGGA1091700.015.1584791
Produced by FastQC (version 0.11.5)