FastQCFastQC Report
Thu 13 Feb 2020
HK5KKDRXX_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5KKDRXX_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492921239
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG10668550.055.567732
AAGCAGT11325150.052.4950181
GCAGTGG11532200.051.338863
AGTGGTA11749550.050.3328365
CAGTGGT13604000.043.628454
GTGGTAT13597700.043.510846
GGTATCA13735100.043.1283078
TGGTATC14652550.040.4446337
GTATCAA14833700.040.0065279
ACGCAGA12881100.026.41864814-15
CAACGCA12975650.026.32287412-13
ATCAACG13631900.025.12358310-11
GCAGAGT13535950.025.10345816-17
AGTACAT13694450.024.62439520-21
CATGGGG9081150.024.3670724-25
AGAGTAC13956400.024.3533618-19
TACATGG13857500.024.29189322-23
GTACATG12811950.022.913922-23
TCAACGC13444700.022.17995812-13
TATCAAC13635450.021.83740210-11
Produced by FastQC (version 0.11.5)