FastQCFastQC Report
Thu 13 Feb 2020
HK5KKDRXX_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5KKDRXX_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492921239
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGCTC7134765814.47445400095653No Hit
AATCTCTC6603178213.396010716430096No Hit
TCGATACT6219403712.617439071234665No Hit
TCAGAAAT6018176512.209205089659363No Hit
AGATCTGG5657629811.477756185709822No Hit
CGGAGGGA5398645910.952349935158708No Hit
GTCTCTCG5235679310.621736061975612No Hit
GTTGAGAA428329618.689615624373612No Hit
GGGGGGGG91169261.8495705355475665No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)