FastQCFastQC Report
Thu 13 Feb 2020
HK5KKDRXX_l01.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK5KKDRXX_l01.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492921239
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGACT1711800.017.7485331
TACCGGG1502500.017.6674961
GTGACGC1541300.017.3590831
CCCGAAG1191100.017.2309761
GAGACCC1205500.017.2122481
CTGTGGG1182750.017.1944851
CGGGAGA741200.017.103684
TGACTAG1287900.016.9595973
ACGCACG1209750.016.8562011
TGCAGGC2429300.016.8479841
TGGGGTG799200.016.809354
AGACACT1638400.016.7755221
ACTGATG1475350.016.6815991
CGAGAGT991100.016.5368526
TTGCTGC1631950.016.5249561
AGCGACT1070400.016.5119678
GATGGGT1127700.016.4982073
TTGATGG1186500.016.4761961
CGGGTGC748950.016.428272
CTGGCAG1552300.016.4117661
Produced by FastQC (version 0.11.5)