FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S8_R1_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S8_R1_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51805
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCG21714.190715181932246No Hit
CTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATGAA17833.4417527265707943No Hit
GTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCG16023.0923656017758905No Hit
AAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGC12372.3878004053662774No Hit
GCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCG11552.2295145256249396No Hit
GGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGT11022.127207798475051No Hit
CCCCAGAAGTTCGCGCAGCACGTAGCCGATGAACAAGGTGTTGCCGACGA10051.9399671846346878No Hit
GCACGTAGCCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGC9601.8531029823376122No Hit
CAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGC9191.7739600424669433No Hit
CCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTG8591.6581411060708424No Hit
GGTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATG8381.6176044783322074No Hit
GTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTCCC7281.4052697616060226No Hit
AAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTC7161.3821059743268023No Hit
CACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCT6501.2547051442910917No Hit
GTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATGAACAAGG6331.221889778978863No Hit
GTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAATGGCTATACC5961.1504681015346008No Hit
GAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTT5911.1408165235015926No Hit
CCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTT5661.0925586333365505No Hit
GCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGC5641.088698002123347No Hit
GAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGC5541.0693948460573304No Hit
AGCAAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCG5411.0443007431715086No Hit
CCCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATG5281.0192066402856867No Hit
GGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTG4970.9593668564810346No Hit
TGGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCT4960.957436540874433No Hit
CACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGC4630.8937361258565776No Hit
CTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCG4630.8937361258565776No Hit
GCGAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGG4510.8705723385773575No Hit
AGCGAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCC4170.8049416079529004No Hit
GCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCC4120.795290029919892No Hit
GAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTG4040.7798475050670785No Hit
GTATAGCCATTCGAGCCCATGGTCACGCATCCATCGTGGCGCCTGGGACT4010.7740565582472735No Hit
CAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCAT3810.7354502461152398No Hit
ATGGATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGC3710.7161470900492231No Hit
AGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGACACAGGCGGTATAGCCA3580.6910529871634011No Hit
GTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTCGTCGGCAACACCTTG3200.6177009941125373No Hit
GTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAG3000.5790946819805038No Hit
GTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGACACAG2900.559791525914487No Hit
CTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCG2830.5462793166682752No Hit
GCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTCG2780.5366277386352669No Hit
AAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCCCCAGA2680.5173245825692501No Hit
GCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTG2630.5076730045362416No Hit
CGATGGATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGA2590.499951742109835No Hit
GAGCGAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTC2570.4960911108966316No Hit
AGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGACAC2550.49223047968342826No Hit
GCTCTTGACACAGGCGGTATAGCCATTCGAGCCCATGGTCACGCATCCAT2530.48836984847022485No Hit
GTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTC2500.4825789016504199No Hit
AGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCC2480.4787182704372165No Hit
CCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATGGATGCGT2460.4748576392240131No Hit
ATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGG2460.4748576392240131No Hit
AGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTG2440.4709970080108098No Hit
GCCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCG2380.45941511437119964No Hit
GGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCG2360.45555448315799635No Hit
GCGAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCG2350.4536241675513947No Hit
GCAAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGG2300.4439725895183862No Hit
GATCGCTGGCACCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGC2080.40150564617314927No Hit
GCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATGGATGCG2060.397645014959946No Hit
CCAGAAGTTCGCGCAGCACGTAGCCGATGAACAAGGTGTTGCCGACGAGG2050.3957146993533443No Hit
GAGCAAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTC1960.3783418588939292No Hit
GCCACAGCCGGCCCGGCTCTTGACACAGGCGGTATAGCCATTCGAGCCCA1960.3783418588939292No Hit
GCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCA1950.37641154328732745No Hit
GGCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATGGATGC1950.37641154328732745No Hit
CACCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAATGGC1880.36289933404111574No Hit
TTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATGAACA1850.3571083872213107No Hit
AAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCC1830.3532477560081073No Hit
GCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATGGATGCGTGACCA1800.3474568091883023No Hit
GGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTC1790.34552649358170057No Hit
GCCCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGAT1760.33973554676189555No Hit
GCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCA1630.31464144387607373No Hit
AACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGT1610.3107808126628704No Hit
GTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGGCGCCACGATGG1540.2972686034166586No Hit
ACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTG1530.29533828781005694No Hit
CACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGC1450.2798957629572435No Hit
AACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTG1440.2779654473506418No Hit
GCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGA1350.2605926068912267No Hit
AGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCG1330.25673197567802336No Hit
GAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGG1330.25673197567802336No Hit
GGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGACACA1280.24708039764501497No Hit
GGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCCCCAGAAGTTC1270.24515008203841326No Hit
GCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCCCCAGAAGTTCG1260.2432197664318116No Hit
GTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTCGTCGGCAACACCT1230.23742881961200654No Hit
CCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGT1230.23742881961200654No Hit
TAGCCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCC1210.23356818839880322No Hit
GCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGACACAGGCGGT1210.23356818839880322No Hit
GAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCC1200.23163787279220152No Hit
GCGCAGCACGTAGCCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCC1150.2219862947591931No Hit
CTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGG1140.22005597915259145No Hit
ACCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAATGGCT1130.21812566354598978No Hit
AGTTCGCGCAGCACGTAGCCGATGAACAAGGTGTTGCCGACGAGGTAGCG1090.21040440111958306No Hit
CCACGATGGATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCA1090.21040440111958306No Hit
GCGCCCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCG1080.20847408551298136No Hit
GCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCAA1070.2065437699063797No Hit
ACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCT1060.204613454299778No Hit
GGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGT1050.20268313869317633No Hit
CGGTATAGCCATTCGAGCCCATGGTCACGCATCCATCGTGGCGCCTGGGA1030.198822507479973No Hit
GACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATGAACAAGGTGTTG1030.198822507479973No Hit
GAAGTTCGCGCAGCACGTAGCCGATGAACAAGGTGTTGCCGACGAGGTAG1010.1949618762667696No Hit
CGCTGGCACCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCG990.19110124505356624No Hit
ACCTTGTTCATCGGCTACGTGCTGCGCGAACTTCTGGGGCGACGTCAGCA960.18531029823376122No Hit
GGCGGTATAGCCATTCGAGCCCATGGTCACGCATCCATCGTGGCGCCTGG930.1795193514139562No Hit
GAGCAAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGC930.1795193514139562No Hit
GGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCT920.1775890358073545No Hit
CTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTCGT920.1775890358073545No Hit
GCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTT910.17565872020075282No Hit
AGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAATGGCTATAC880.16986777338094777No Hit
GCTACGTGCTGCGCGAACTTCTGGGGCGACGTCAGCACTAAAGGGCGCGG880.16986777338094777No Hit
CGCCCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGA870.1679374577743461No Hit
CCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGG850.16407682656114275No Hit
AAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCG830.1602161953479394No Hit
GAGCGAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGC770.1486343017083293No Hit
ATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCTACCTCGTCG770.1486343017083293No Hit
GGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGG770.1486343017083293No Hit
GGATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCG750.14477367049512596No Hit
CGCCACGATGGATGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGT740.1428433548885243No Hit
ACGTAGCCGATGAACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCG720.1389827236753209No Hit
GTCACGCATCCATCGTGGCGCCTGGGACTTGGTGCCAGCGATCAGCGCAA720.1389827236753209No Hit
TGCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGGC720.1389827236753209No Hit
GCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATGAACAAGGTG720.1389827236753209No Hit
CATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCAAGTCCCAGG700.13512209246211757No Hit
CCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCG700.13512209246211757No Hit
CGCCACCTGAACGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGAT680.1312614612489142No Hit
TCTTGACACAGGCGGTATAGCCATTCGAGCCCATGGTCACGCATCCATCG680.1312614612489142No Hit
CAGCCGGCCCGGCTCTTGACACAGGCGGTATAGCCATTCGAGCCCATGGT680.1312614612489142No Hit
CGAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGT680.1312614612489142No Hit
GTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGACACAGGC670.12933114564231252No Hit
CGAATGGCTATACCGCCTGTGTCAAGAGCCGGGCCGGCTGTGGCGGCGCT670.12933114564231252No Hit
GCAAAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCG660.12740083003571084No Hit
CATCCATCGTGGCGCCTGGGACTTGGTGCCAGCGATCAGCGCAAAGGCCA660.12740083003571084No Hit
ACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCCGCGCCC660.12740083003571084No Hit
ACAAGGTGTTGCCGACGAGGTAGCGCCGCCACAGCCGGCCCGGCTCTTGA640.12354019882250748No Hit
AAAGCAGGAGTTCTGGATGGAAGCGCACCAGCGCCACCTGAACGCCGTGT620.11967956760930412No Hit
CGCCGTGTCCATCGGCGTCGGTCTGGCCTTTGCGCTGATCGCTGGCACCA620.11967956760930412No Hit
AGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGCCGCC600.11581893639610076No Hit
CCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGCAGCACGTAGCCGATGA590.11388862078949909No Hit
ATTCGAGCCCATGGTCACGCATCCATCGTGGCGCCTGGGACTTGGTGCCA590.11388862078949909No Hit
GGCACCAAGTCCCAGGCGCCACGATGGATGCGTGACCATGGGCTCGAATG580.1119583051828974No Hit
GCGTGACCATGGGCTCGAATGGCTATACCGCCTGTGTCAAGAGCCGGGCC580.1119583051828974No Hit
GATGGAAGCGCACCAGCGCCACCTGAACGCCGTGTCCATCGGCGTCGGTC580.1119583051828974No Hit
CGGCCAAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCCCCAGAAGTT560.10809767396969404No Hit
AAGTCGGCCGCCGCGCCCTTTAGTGCTGACGTCGCCCCAGAAGTTCGCGC530.102306727149889No Hit
AGCCATTCGAGCCCATGGTCACGCATCCATCGTGGCGCCTGGGACTTGGT520.10037641154328732No Hit
CGAAAGCAGCACACCCGGCGGGCAAACTGCGGGAAATCGGCCAAGTCGGC520.10037641154328732No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)