FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S8_R1_H11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S8_R1_H11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239258
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAA144066.0211152814116975No Hit
ATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATA68372.8575846993621945No Hit
ATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATACT62682.6197661102241097No Hit
ATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCT49782.080599185816148No Hit
GTTATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTC49302.0605371607218985No Hit
ATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAA41931.7525014837539394No Hit
CCTTTAAAGAGGCCAAGTAAATAACCCAGAGTCATTCACTAGGAAGTGGC41711.7433063889190747No Hit
AAACAATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCAC32781.370069130394804No Hit
CTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACATT32121.3424838458902104No Hit
ATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATG31831.3303630390624348No Hit
CTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCAGAGTC30621.2797900174706802No Hit
CTTCTTGTCTGTTCATTTTCTTACATTCCACATTCATATTTCCTGCTTTT29601.2371582141453996No Hit
GGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATA28881.207065176504025No Hit
ATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGTTTT26561.1100987218818181No Hit
ATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACATTCA26361.101739544759214No Hit
ATTATGATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAACCTTTA25501.0657950831320164No Hit
ACTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTAT25231.054510194016501No Hit
GACAAGAAGAATCAAAGTTATATAGGATTATAAACTTTGTCTTAGCCTCA24881.039881634051944No Hit
ATCCTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCAC24531.0252530740873869No Hit
GTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATACTGAGTAGTGGTAA20980.876877680161165No Hit
GTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGG20010.8363356711165353No Hit
AAATAACCCAGAGTCATTCACTAGGAAGTGGCAGAATTATAATTAAACTC18390.7686263364234425No Hit
GATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAG17650.7376973810698075No Hit
GGGCACTATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAA17470.7301741216594638No Hit
AGCGAAAGCAGGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGA16770.7009170017303497No Hit
TAATTATGATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAACCTT16690.697573330881308No Hit
AAGTTATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCT16410.6858704829096623No Hit
GTCTGGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAA16330.6825268120606207No Hit
GATTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATAC15560.6503439801385952No Hit
ACCCAGAGTCATTCACTAGGAAGTGGCAGAATTATAATTAAACTCCATTT15270.6382231733108192No Hit
ACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAA15070.6298639961882152No Hit
GTAAATAACCCAGAGTCATTCACTAGGAAGTGGCAGAATTATAATTAAAC14410.6022787116836219No Hit
GTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGG14380.6010248351152312No Hit
ACTCAGTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTAAGACAAAGTTTA13600.5684240443370754No Hit
GAAAGCAGGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAG13420.5609007849267318No Hit
AAGCAGGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAGAG13180.550869772379607No Hit
GGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGG12740.532479582709878No Hit
CTGCAGGACCGTGATCTTATGGTGCTGGGATTGAGCCCCCTCTCGGGCTT12500.5224485701627533No Hit
ACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAG12380.5174330638891907No Hit
ATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGGAATGTAAGAA11650.4869220673916859No Hit
GTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTAAGACAAAGTTTATAATC11490.48023472569360276No Hit
TATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCAGAGTCATTCA11390.4760551371323007No Hit
CCTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACAT10760.44972372919609793No Hit
ATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTACAAAA10660.44554414063479586No Hit
GATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTACAAA10270.429243745245718No Hit
CTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACATTCATATTTC10040.4196306915547234No Hit
CACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCAGAG9370.39162744819399975No Hit
GAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAGTGA9120.38117847679074474No Hit
TTCTTACATTCCACATTCATATTTCCTGCTTTTTTTCCTTTTGTCCAAAT8950.37407317623653125No Hit
TTCATATTTCCTGCTTTTTTTCCTTTTGTCCAAATAGACCAAAACATAGT8320.3477417683003285No Hit
GAATCAAAGTTATATAGGATTATAAACTTTGTCTTAGCCTCAAGAAGAAA8210.3431442208828963No Hit
CTTTAAAGGTTTCCCCAAATATGAAGTGAGGATAATAGTGCCCAAATCAT8130.3398005500338547No Hit
GTTCATTTTCTTACATTCCACATTCATATTTCCTGCTTTTTTTCCTTTTG8030.3356209614725526No Hit
TTTTCTTACATTCCACATTCATATTTCCTGCTTTTTTTCCTTTTGTCCAA7720.3226642369325164No Hit
ACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAA7640.31932056608347476No Hit
GGATAATAGTGCCCAAATCATAATTATTTTTATTTTTTTTTAAGATTTTT7620.3184846483712144No Hit
GTCATTCACTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACCAC7560.3159768952344331No Hit
AAATAATTATGATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAAC7340.30678180039956865No Hit
GAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCA7320.30594588268730827No Hit
TTATAATTCTGCCACTTCCTAGTGAATGACTCTGGGTTATTTACTTGGCC7170.2996764998453552No Hit
GGAAATATGAATGTGGAATGTAAGAAAATGAACAGACAAGAAGAATCAAA6920.28922752844210015No Hit
CATATTTCCTGCTTTTTTTCCTTTTGTCCAAATAGACCAAAACATAGTAT6700.2800324336072357No Hit
ATCTTATGGTGCTGGGATTGAGCCCCCTCTCGGGCTTCCTGCTCAGGGGG6360.2658218324988088No Hit
GTTTATAATCCTATATAACTTTGATTCTTCTTGTCTGTTCATTTTCTTAC6260.2616422439375068No Hit
CTATGGGGGCACCTGCAGGACCGTGATCTTATGGTGCTGGGATTGAGCCC6180.25829857308846516No Hit
CAATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTC6080.25411898452716314No Hit
GTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAGTGAATGACTCT6060.2532830668149027No Hit
GAAGAAAAGGCCTTCAAACAATACTGAGTAGTGGTAAAAATGGAGTTTAA5990.25035735482199134No Hit
GAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAA5990.25035735482199134No Hit
CCTTCAAACAATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCT5900.24659572511681951No Hit
GTTTAATTATAATTCTGCCACTTCCTAGTGAATGACTCTGGGTTATTTAC5790.24199817769938728No Hit
ACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATACTGAGTAGT5650.23614675371356444No Hit
GTAAGAAAATGAACAGACAAGAAGAATCAAAGTTATATAGGATTATAAAC5640.23572879485743423No Hit
GGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGGAATGT5570.2328030828645228No Hit
ATATGAATGTGGAATGTAAGAAAATGAACAGACAAGAAGAATCAAAGTTA5510.2302953297277416No Hit
CTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATT5450.22778757659096038No Hit
CACTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACCACTACTCA5400.22569778231030937No Hit
CATTCATATTTCCTGCTTTTTTTCCTTTTGTCCAAATAGACCAAAACATA5380.22486186459804897No Hit
ACTTTGATTCTTCTTGTCTGTTCATTTTCTTACATTCCACATTCATATTT5350.22360798802965837No Hit
ATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACC5270.22026431718061676No Hit
ATCTCATTTAATATTTAAAATAACTATGGGGGCACCTGCAGGACCGTGAT5270.22026431718061676No Hit
CTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACCACTACTCAGT5140.2148308520509241No Hit
GGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAGTGAATGACTC5120.21399493433866373No Hit
GAAAAGGCCTTCAAACAATACTGAGTAGTGGTAAAAATGGAGTTTAATTA5090.21274105777027308No Hit
CTTACATTCCACATTCATATTTCCTGCTTTTTTTCCTTTTGTCCAAATAG4950.20688963378445024No Hit
CTACTCAGTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTAAGACAAAGTT4930.20605371607218984No Hit
ATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCAGAGTCATTC4850.20271004522314823No Hit
GCAGGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAGAGAG4820.20145616865475763No Hit
CAAGAAGAAAAGGCCTTCAAACAATACTGAGTAGTGGTAAAAATGGAGTT4720.1972765800934556No Hit
GCCTCAAGAAGAAAAGGCCTTCAAACAATACTGAGTAGTGGTAAAAATGG4680.19560474466893482No Hit
ACTCTGGGTTATTTACTTGGCCTCTTTAAAGGTTTCCCCAAATATGAAGT4660.19476882695667438No Hit
CTCCTACCCTTCCCCTCCTCAAATAAATAAAAATCTTAAAAAAAAATAAA4600.19226107381989316No Hit
AACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATACTGAGTAG4600.19226107381989316No Hit
GGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTAC4540.18975332068311196No Hit
TAATTAAACTCCATTTTTACCACTACTCAGTATTGTTTGAAGGCCTTTTC4520.18891740297085155No Hit
GCACTATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGT4510.18849944411472136No Hit
AATTATAATTCTGCCACTTCCTAGTGAATGACTCTGGGTTATTTACTTGG4500.18808148525859114No Hit
GAATTATAATTAAACTCCATTTTTACCACTACTCAGTATTGTTTGAAGGC4500.18808148525859114No Hit
GGACTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCT4470.18682760869020054No Hit
TTATAAACTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATACTG4440.18557373212180994No Hit
CATTTAATATTTAAAATAACTATGGGGGCACCTGCAGGACCGTGATCTTA4420.18473781440954953No Hit
GTGCTGGGATTGAGCCCCCTCTCGGGCTTCCTGCTCAGGGGGAGTCTGCT4420.18473781440954953No Hit
GTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAGTGAAT4410.18431985555341932No Hit
GAGGATAATAGTGCCCAAATCATAATTATTTTTATTTTTTTTTAAGATTT4390.1834839378411589No Hit
GCGAAAGCAGGAGATTAAAAACAAATTTAGGACTTAGAAGTCTGGGAGAG4320.1805582258482475No Hit
GAGTCATTCACTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACC4300.1797223081359871No Hit
AGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAA4290.1793043492798569No Hit
ATGTAAGAAAATGAACAGACAAGAAGAATCAAAGTTATATAGGATTATAA4140.17303496643790384No Hit
TGTCTGTTCATTTTCTTACATTCCACATTCATATTTCCTGCTTTTTTTCC4120.17219904872564346No Hit
CAAACAATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCA4050.16927333673273204No Hit
GGCCAAGTAAATAACCCAGAGTCATTCACTAGGAAGTGGCAGAATTATAA4020.1680194601643414No Hit
CTATCTCATTTAATATTTAAAATAACTATGGGGGCACCTGCAGGACCGTG4000.16718354245208103No Hit
CTGGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAA3970.1659296658836904No Hit
TATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAAC3950.16509374817143No Hit
TTTGGGCACTATTATCCTCACTTCATATTTGGGGAAACCTTTAAAGAGGC3920.1638398716030394No Hit
CCTCACTTCATATTTGGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCA3910.1634219127469092No Hit
GGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTATTTGGACAAAAGGA3890.1625859950346488No Hit
GGGTAGGAGGGAGAAGCAGACTCCCCCTGAGCAGGAAGCCCGAGAGGGGG3850.16091415961012798No Hit
GTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTTCCTAGTGAATGAC3810.1592423241856072No Hit
CTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGTGGAATGTAAG3780.15798844761721656No Hit
TATGTACACTATCTCATTTAATATTTAAAATAACTATGGGGGCACCTGCA3770.15757048876108634No Hit
AAATATGAATGTGGAATGTAAGAAAATGAACAGACAAGAAGAATCAAAGT3710.15506273562430514No Hit
TTCTTCTTGTCTGTTCATTTTCTTACATTCCACATTCATATTTCCTGCTT3590.15004722935074272No Hit
GTGAATGACTCTGGGTTATTTACTTGGCCTCTTTAAAGGTTTCCCCAAAT3580.1496292704946125No Hit
AAATACTATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATA3520.1471215173578313No Hit
GTCCTGCAGGTGCCCCCATAGTTATTTTAAATATTAAATGAGATAGTGTA3510.1467035585017011No Hit
AAAATGAACAGACAAGAAGAATCAAAGTTATATAGGATTATAAACTTTGT3500.1462855996455709No Hit
CCACTACTCAGTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTAAGACAAA3490.1458676407894407No Hit
GGGTTATTTACTTGGCCTCTTTAAAGGTTTCCCCAAATATGAAGTGAGGA3490.1458676407894407No Hit
ATGTTTTGGTCTATTTGGACAAAAGGAAAAAAAGCAGGAAATATGAATGT3480.14544968193331048No Hit
ATTCACTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACCACTAC3470.14503172307718026No Hit
AATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGTTT3460.14461376422105007No Hit
GAACAGACAAGAAGAATCAAAGTTATATAGGATTATAAACTTTGTCTTAG3420.14294192879652928No Hit
GGGGAAGGGTAGGAGGGAGAAGCAGACTCCCCCTGAGCAGGAAGCCCGAG3280.13709050481070642No Hit
ATTATAATTCTGCCACTTCCTAGTGAATGACTCTGGGTTATTTACTTGGC3270.13667254595457623No Hit
TATTTGAGGAGGGGAAGGGTAGGAGGGAGAAGCAGACTCCCCCTGAGCAG3200.1337468339616648No Hit
AATATGAAGTGAGGATAATAGTGCCCAAATCATAATTATTTTTATTTTTT3180.1329109162494044No Hit
ACAATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACTT3160.132074998537144No Hit
CGTCCACACCGGCCGCGCTCAGCCCGATGCCGATCGAGGCGCCCAGCAGA3130.1308211219687534No Hit
GATCATGACCTGAGCCAAAGGCAGACACTAACCGACTAGGCCACCCAGAT3110.129985204256493No Hit
GGGGAAACCTTTAAAGAGGCCAAGTAAATAACCCAGAGTCATTCACTAGG3000.12538765683906078No Hit
CAAGAAGAATCAAAGTTATATAGGATTATAAACTTTGTCTTAGCCTCAAG2960.12371582141453995No Hit
ATTTTTACCACTACTCAGTATTGTTTGAAGGCCTTTTCTTCTTGAGGCTA2940.12287990370227955No Hit
CCCTTCCCCTCCTCAAATAAATAAAAATCTTAAAAAAAAATAAAAATAAT2910.12162602713388894No Hit
ATTATAATTAAACTCCATTTTTACCACTACTCAGTATTGTTTGAAGGCCT2900.12120806827775875No Hit
GGCAGAGGGAGAGGGACAAGCTGGCTTCCTGATGAGCAAAAGACCCCCCT2860.11953623285323792No Hit
AATTATGATTTGGGCACTATTATCCTCACTTCATATTTGGGGAAACCTTT2860.11953623285323792No Hit
GAATGTAAGAAAATGAACAGACAAGAAGAATCAAAGTTATATAGGATTAT2860.11953623285323792No Hit
ACTATGGGGGCACCTGCAGGACCGTGATCTTATGGTGCTGGGATTGAGCC2840.11870031514097752No Hit
CAAGTAAATAACCCAGAGTCATTCACTAGGAAGTGGCAGAATTATAATTA2820.11786439742871711No Hit
GTTATTTACTTGGCCTCTTTAAAGGTTTCCCCAAATATGAAGTGAGGATA2770.1157746031480661No Hit
GCTCAGGGGGAGTCTGCTTCTCCCTCCTACCCTTCCCCTCCTCAAATAAA2740.11452072657967549No Hit
TCCCCCATGTGGGGCTCGATCCCAGGACCCTCAGATCATGACCTGAGCCA2700.11284889115515469No Hit
GATTCTTCTTGTCTGTTCATTTTCTTACATTCCACATTCATATTTCCTGC2690.11243093229902448No Hit
GCAGGGCACCGTCCGCGCACTCAACGCGCAGGAGCTGGGCCACCTCAAGG2680.11201297344289429No Hit
AGCAGGAGGAGCAGCAGGCAGAGGGAGAGGGACAAGCTGGCTTCCTGATG2590.10825134373772245No Hit
CTTTGTCTTAGCCTCAAGAAGAAAAGGCCTTCAAACAATACTGAGTAGTG2560.10699746716933187No Hit
CAAATTTAGGACTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGT2560.10699746716933187No Hit
AACTATGGGGGCACCTGCAGGACCGTGATCTTATGGTGCTGGGATTGAGC2530.10574359060094125No Hit
CCCATGTGGGGCTCGATCCCAGGACCCTCAGATCATGACCTGAGCCAAAG2520.10532563174481104No Hit
CCATTTTTACCACTACTCAGTATTGTTTGAAGGCCTTTTCTTCTTGAGGC2520.10532563174481104No Hit
TTCCACATTCATATTTCCTGCTTTTTTTCCTTTTGTCCAAATAGACCAAA2500.10448971403255064No Hit
GGCTAAGACAAAGTTTATAATCCTATATAACTTTGATTCTTCTTGTCTGT2500.10448971403255064No Hit
GACTTAGAAGTCTGGGAGAGAGAGAGTACAAAATACTATGTTTTGGTCTA2470.10323583746416003No Hit
ACTAGGAAGTGGCAGAATTATAATTAAACTCCATTTTTACCACTACTCAG2450.10239991975189962No Hit
AAGAAAAGGCCTTCAAACAATACTGAGTAGTGGTAAAAATGGAGTTTAAT2440.10198196089576941No Hit
AACAATACTGAGTAGTGGTAAAAATGGAGTTTAATTATAATTCTGCCACT2430.10156400203963922No Hit
CCCTTCCCCCATGTGGGGCTCGATCCCAGGACCCTCAGATCATGACCTGA2400.10031012547124861No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)