FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S8_R1_G10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S8_R1_G10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968246
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGTAGGTAAACCTCTAGCTTTAATCTTTTGCCATCTCTGATCTC53070.26963093028005647No Hit
GTCTTAAGTACTATTGCTTTATAGTAAGTTTTGAAATCAGGAAGTGGGAA52820.2683607638476085No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT44640.2268009181779107No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG42920.21806217312266862No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT34200.17375876795888318No Hit
ACTTAAGACAGTGTGCAAGTGTCACAAGGATAAACAAACAGATTAACAGC32470.1649692162463432No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA30370.1542998182137802No Hit
ATATTAGAGAAGATCTAAATAATTGGAAAAAACATCCTGTGTTCATGGAC26920.1367715214459981No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA23300.11837951150415141No Hit
GATCTAAATAATTGGAAAAAACATCCTGTGTTCATGGACTGGAAGACAAC22140.11248593925759282No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)