FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S8_R1_G09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S8_R1_G09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088757
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTACTGTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTT81800.39162047093079755No Hit
CTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTA54810.2624048656689122No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG54240.2596759699668272No Hit
ATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTGTTGTACAA54080.25890996415571554No Hit
ATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATGATC46000.22022667069458057No Hit
GTACTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTA42210.2020819080438749No Hit
GTTAAGTACATTCATTGTTGTACAACCTCCAAAACTTTTTCATCTTGTGA41580.19906576016262303No Hit
GTGTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCC38970.1865702903688653No Hit
GATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAATAGCTAAAAG32110.1537277912174561No Hit
GTTCATAGCAGCATTCCTCCAAATAGCTAAAAGGTTAATCAACCCAAATG31760.15205215350564955No Hit
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT29030.13898217935355814No Hit
CCTTAATCATTCTAAAGTGTACGGTTTAGTACTGTTAAGTACATTCATTG26700.12782721972924566No Hit
GAGCAAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCA25960.12428444285285459No Hit
CTATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAA25100.12016716161812983No Hit
GTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTTACTTCACATATG24740.11844364854312878No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA24540.11748614127923929No Hit
GATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTCCTCCAAA24270.11619350647298848No Hit
CTATTCTACTTTGTGTTTCTAGGAATCTGATTACTCTAAGTACCTTATGT23710.11351248613409794No Hit
ATATGACACTACTTACATAAGGTACTTAGAGTAATCAGATTCCTAGAAAC22100.10580455265978761No Hit
ACCCAAATGTGCTCTGATGATGAATGGATAAACAAAACATGGTGTATACA21760.1041767903111755No Hit
AAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCATAGCAGCATTC21710.10393741349520313No Hit
TATGTAAGTAGTGTCATATAGTACTTGTCGTTTGACAACTAGCTTATTTT21110.10106489170353468No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)