FastQCFastQC Report
Sat 1 May 2021
HK3VMBGXH_n02_S8_R1_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK3VMBGXH_n02_S8_R1_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919953
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGT219071.1410175144912402No Hit
TCTTTATCTCGCTCACAGGAAAGGTGTCGCCCCTCTGGCAGGTGGGCAGG78970.411312151912052No Hit
CCCTAAACACTGTGGAGATGCTGCGAGTAGCAAGCTCTTCTCTGGGCATG75220.3917804237916241No Hit
TCATAAACCGGTGGCACTTCCCACAGCGGGACAGGGGCTTGCCCGTGGCC74330.38714489365104254No Hit
GTGCAGGCGGCTCGGCTTGGCCTGGATGTACTTCATAAACCGGTGGCACT68730.3579775129912034No Hit
GTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGA68710.35787334377456115No Hit
GTGTGAGCAGTGCAGGCGGCTCGGCTTGGCCTGGATGTACTTCATAAACC64760.3372999234877104No Hit
GTATGAGTACATCACCAGACACTTCATCGCCACGGTGAGCTACGACTGCA64010.3333935778636248No Hit
ATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCG62510.3255808866154536No Hit
GACGAGTACTGCGTGCAGCAACTCAAGGAGTTTGATGGGAAGAGTCTAGT60900.3171952646757499No Hit
ATCTTGTAGTAGCCATGCACCAGGACGATGCCCAGGTTGGTGGGCTTGAG60090.31297641140173743No Hit
GGCCTAGCTTAATCATGCGGTAGATGCGGTTGGAGTGAGTCTGGGGATCC53240.27729845470175574No Hit
TCCCACAGCGGGACAGGGGCTTGCCCGTGGCCGCCAGGGGCGAAAAAGAC51070.26599609469606805No Hit
TTCATGATCCGCTCCATGTTGGCTGTCCAGCCATAGGTGCTAGTCACAAT49540.2580271496229335No Hit
GCCCAGGACCTGGCGGTAGTCAGCCTTGCCCTGGGCGATCAGGTTTAGCT48190.25099572749957944No Hit
AGATAAAGATGCTGGAGAAACAGACGAGCCCGCCCGACTACCTGACTGAG45940.23927669062732262No Hit
GTCCCAATGCCATGCTTCTCCATGAGTGTGATGAGCTCAGCCTCAGTCAG45740.23823499846089982No Hit
TCACCAGACACTTCATCGCCACGGTGAGCTACGACTGCAAGTACCTCCAG44550.23203693007068402No Hit
TCCCTGTGCACATCAACAACATCTGTCAACGCAACTATGTCACTGTGGAG42770.2227658697895209No Hit
CACCAGGACGATGCCCAGGTTGGTGGGCTTGAGCCGGCGCCCACTCTCCA41700.21719281669915877No Hit
GTAAAGCGCTTGTTAGCAGAAGGTATCAACCGGCCACGGAAGGGCCACGA40200.2093801254509876No Hit
GCCTTGCCCTGGGCGATCAGGTTTAGCTGCTTCTCGACTGCACTGCGGAT39160.20396332618558893No Hit
ATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGTTT38720.20167160341945872No Hit
GAATTAGGGGGCGAGGCATGGCGGCTGTATGAGTACATCACCAGACACTT38410.2000569805615033No Hit
GTCCTGGTGCATGGCTACTACAAGATTGATGCAGAGCTGGTGCTCCCCAC38190.19891111917843823No Hit
GTACTGGGCTGACACAGTAAAGCGCTTGTTAGCAGAAGGTATCAACCGGC38040.19812985005362108No Hit
GCTCTACACACAAGGCTACATCAGCTACCCGCGGACCGAGACCACCCACT38030.19807776544529995No Hit
GTACATCACCAGACACTTCATCGCCACGGTGAGCTACGACTGCAAGTACC38010.19797359622865768No Hit
CAGTGTGAGCAGTGCAGGCGGCTCGGCTTGGCCTGGATGTACTTCATAAA37990.1978694270120154No Hit
CTACAAGATTGATGCAGAGCTGGTGCTCCCCACCATCCGCAGTGCAGTCG36530.1902650741971288No Hit
TCTCTGGGCATGGGGCCTCAGCATGCCATGCAGACCGCCGAGCGGCTCTA36180.18844211290588883No Hit
CTCATGGAGAAGCATGGCATTGGGACAGACGCCAGCATCCCTGTGCACAT35780.1863587285730432No Hit
GTAGTGAAACTTCCTCTTGAAGACATCCAGTGTGTGGCCCAGGACCTGGC35730.1860983055314375No Hit
CCTCCAGAGGCACGCTCTGCCAGGGCATGATCTCCGTGAAGCCTGGAGAG35110.18286905981552673No Hit
AGTGTGAGCAGTGCAGGCGGCTCGGCTTGGCCTGGATGTACTTCATAAAC34680.18062942165771767No Hit
GTAGTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTCAA34150.17786893741669718No Hit
GCACAGGGATGCTGGCGTCTGTCCCAATGCCATGCTTCTCCATGAGTGTG33780.17594180690881495No Hit
GTCACAGTGTGAGCAGTGCAGGCGGCTCGGCTTGGCCTGGATGTACTTCA33110.1724521381512985No Hit
CTCCAGGCTTCACGGAGATCATGCCCTGGCAGAGCGTGCCTCTGGAGGAG32870.1712021075515911No Hit
CAGGAAAGGTGTCGCCCCTCTGGCAGGTGGGCAGGCTCTCCTCCAGAGGC32600.1697958231269203No Hit
GTGGAGAGTGGGCGCCGGCTCAAGCCCACCAACCTGGGCATCGTCCTGGT32480.16917080782706662No Hit
GTGACATAGTTGCGTTGACAGATGTTGTTGATGTGCACAGGGATGCTGGC32470.16911872321874546No Hit
CCCATGAGGTCTGCCACTGAGGCCGAATTAGGGGGCGAGGCATGGCGGCT32260.16802494644400148No Hit
TTGTTGATGTGCACAGGGATGCTGGCGTCTGTCCCAATGCCATGCTTCTC31410.1635977547367045No Hit
TCTCGTCACAGTGTGAGCAGTGCAGGCGGCTCGGCTTGGCCTGGATGTAC31380.1634415009117411No Hit
TCATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATC31240.16271231639524508No Hit
CTCCATGAGTGTGATGAGCTCAGCCTCAGTCAGGTAGTCGGGCGGGCTCG31180.16239980874531826No Hit
CTGTGGAGATGCTGCGAGTAGCAAGCTCTTCTCTGGGCATGGGGCCTCAG30750.16016017058750917No Hit
GGTTTAGCTGCTTCTCGACTGCACTGCGGATGGTGGGGAGCACCAGCTCT30650.15963932450429777No Hit
GTCCCGAAGGGCCTGGGCTTTCATGATCCGCTCCATGTTGGCTGTCCAGC30620.15948307067933434No Hit
GTACTCATACAGCCGCCATGCCTCGCCCCCTAATTCGGCCTCAGTGGCAG30320.1579205324297001No Hit
GTCTAGTCTCTGTTACCAAGGAGGGCCTGGAGCTGCCTGAGGATGAAGAG30030.156410078788387No Hit
GCCTGAGGATGAAGAGGAGAAAAAGAAAATGGAGGAGAGCAAGGCCAAGT29960.15604548653013903No Hit
AGCTTAATCATGCGGTAGATGCGGTTGGAGTGAGTCTGGGGATCCTCCAG29530.15380584837232994No Hit
CTGTTACCAAGGAGGGCCTGGAGCTGCCTGAGGATGAAGAGGAGAAAAAG29140.15177454864780546No Hit
GTCTGGGGATCCTCCAGTGAGAAGCCAGAAGAGAGCAGAGCTGTTTCAAA29010.1510974487396306No Hit
CATCACACTCATGGAGAAGCATGGCATTGGGACAGACGCCAGCATCCCTG28680.14937865666503294No Hit
CTCCACAGTGACATAGTTGCGTTGACAGATGTTGTTGATGTGCACAGGGA28630.14911823362342724No Hit
CCTTTCCTGTGAGCGAGATAAAGATGCTGGAGAAACAGACGAGCCCGCCC28230.1470348492905816No Hit
GTGGAGATGCTGCGAGTAGCAAGCTCTTCTCTGGGCATGGGGCCTCAGCA28120.14646191859904906No Hit
GGATGTACTTCATAAACCGGTGGCACTTCCCACAGCGGGACAGGGGCTTG28070.14620149555744333No Hit
GTCCTTGACAGCTTTGTCGTTCTTGTCCGCCTCTGCCTTCTGCCGCAGCG27700.14427436504956112No Hit
CTTCACCTGCACAGGCAAAACCGTCATCTCTCCAGGCTTCACGGAGATCA27490.14318058827481714No Hit
CATCAACAACATCTGTCAACGCAACTATGTCACTGTGGAGAGTGGGCGCC27470.14307641905817486No Hit
CTCCAGAGCACCATCGCCTTCAGAATTGGGTCCGAGCACTTCACCTGCAC27250.14193055767510976No Hit
CCATTACCCCCATGAGGTCTGCCACTGAGGCCGAATTAGGGGGCGAGGCA27170.14151388080854063No Hit
CATCTAGGTCTAGCCTCAAGGCTGGGTACCTCCCAAATGGCCAAGGAAGG26920.1402117656005121No Hit
GTCTGGTGATGTACTCATACAGCCGCCATGCCTCGCCCCCTAATTCGGCC26920.1402117656005121No Hit
GCATTGGGACAGACGCCAGCATCCCTGTGCACATCAACAACATCTGTCAA26860.13989925795058525No Hit
CCATCAGCTCATCCATGCCAGCGATAGAGTCCACGAAGTAGTGAAACTTC26810.13963883490897955No Hit
GGAAAGGTGTCGCCCCTCTGGCAGGTGGGCAGGCTCTCCTCCAGAGGCAC26700.139065904217447No Hit
GGGCTATATGATGGCCAAGAAGCACCTGGAGATCAATCCTGACCACCCCA26630.138701311959199No Hit
CCAGAGCACCATCGCCTTCAGAATTGGGTCCGAGCACTTCACCTGCACAG26580.1384408889175933No Hit
GCTTAATCATGCGGTAGATGCGGTTGGAGTGAGTCTGGGGATCCTCCAGT26510.13807629665934532No Hit
CTTCATCGCCACGGTGAGCTACGACTGCAAGTACCTCCAGAGCACCATCG26490.13797212744270304No Hit
GGACAGGGGCTTGCCCGTGGCCGCCAGGGGCGAAAAAGACACCTCCATCA26420.13760753518445504No Hit
GCCTATTGACGAGTACTGCGTGCAGCAACTCAAGGAGTTTGATGGGAAGA26410.13755545057613391No Hit
TTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCGCTCCATGTTG26400.13750336596781276No Hit
GAATTGGGTCCGAGCACTTCACCTGCACAGGCAAAACCGTCATCTCTCCA26170.1363054199764265No Hit
GTCCACGAAGTAGTGAAACTTCCTCTTGAAGACATCCAGTGTGTGGCCCA26170.1363054199764265No Hit
CATATAGCCCATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCC26120.1360449969348208No Hit
CAGTAAAGCGCTTGTTAGCAGAAGGTATCAACCGGCCACGGAAGGGCCAC26030.13557623545993053No Hit
GGCAAAACCGTCATCTCTCCAGGCTTCACGGAGATCATGCCCTGGCAGAG25960.13521164320168255No Hit
TTTGTGGAGCGAGTGCGAAAGCGGGGCTTCGAGGTAGTATATATGACAGA25920.13500330476839797No Hit
GTACTGCGTGCAGCAACTCAAGGAGTTTGATGGGAAGAGTCTAGTCTCTG25830.1345345432935077No Hit
CACCTGCACAGGCAAAACCGTCATCTCTCCAGGCTTCACGGAGATCATGC25630.13349285112708487No Hit
GCTCTGCATCAATCTTGTAGTAGCCATGCACCAGGACGATGCCCAGGTTG25390.1322428205273775No Hit
GCCCAGGCCTAGCTTAATCATGCGGTAGATGCGGTTGGAGTGAGTCTGGG25380.13219073591905636No Hit
CTATGTCACTGTGGAGAGTGGGCGCCGGCTCAAGCCCACCAACCTGGGCA25260.13156572061920266No Hit
GCCCTGGGCGATCAGGTTTAGCTGCTTCTCGACTGCACTGCGGATGGTGG25240.13146155140256036No Hit
GTCTTCACCCTGCTGCATTGTGACTAGCACCTATGGCTGGACAGCCAACA25210.13130529757759696No Hit
AGTGTGATGAGCTCAGCCTCAGTCAGGTAGTCGGGCGGGCTCGTCTGTTT25160.13104487453599126No Hit
GTGCAGGTGAAGTGCTCGGACCCAATTCTGAAGGCGATGGTGCTCTGGAG25150.1309927899276701No Hit
ATTGTGGAGTTGTCCCGAAGGGCCTGGGCTTTCATGATCCGCTCCATGTT24660.1284406441199342No Hit
GATCATGCCCTGGCAGAGCGTGCCTCTGGAGGAGAGCCTGCCCACCTGCC24300.1265655982203731No Hit
GCCCTAAACACTGTGGAGATGCTGCGAGTAGCAAGCTCTTCTCTGGGCAT24250.1263051751787674No Hit
GGTCAGGATTGATCTCCAGGTGCTTCTTGGCCATCATATAGCCCATTGTG24210.12609683674548283No Hit
GACTGAGGCTGAGCTCATCACACTCATGGAGAAGCATGGCATTGGGACAG24010.12505514457906No Hit
GGTATCAACCGGCCACGGAAGGGCCACGACGCTGGTGACCATCCCCCCAT23870.12432596006256404No Hit
GGCCTGGATGTACTTCATAAACCGGTGGCACTTCCCACAGCGGGACAGGG23370.12172172964650697No Hit
CATCCAGTGTGTGGCCCAGGACCTGGCGGTAGTCAGCCTTGCCCTGGGCG23350.12161756042986469No Hit
CCACAATGGGGTGGTCAGGATTGATCTCCAGGTGCTTCTTGGCCATCATA23270.12120088356329554No Hit
GGATTGATCTCCAGGTGCTTCTTGGCCATCATATAGCCCATTGTGGAGTT23110.1203675298301573No Hit
CCATGAGTGTGATGAGCTCAGCCTCAGTCAGGTAGTCGGGCGGGCTCGTC22730.11838831471395395No Hit
GCATCAATCTTGTAGTAGCCATGCACCAGGACGATGCCCAGGTTGGTGGG22640.11791955323906367No Hit
GTTAGAGATGGTCACCTTCTCAACCTTCTTATCCAAGATTTCTTTCATGA22530.11734662254753112No Hit
AGGTAGTATATATGACAGAGCCTATTGACGAGTACTGCGTGCAGCAACTC22390.11661743803103514No Hit
CCCTAATTCGGCCTCAGTGGCAGACCTCATGGGGGTAATGGGGGGATGGT22180.11552366125629117No Hit
GGAAAGAGAAGGCCAAGCAGCGGCCCCTCGCCCTAAACACTGTGGAGATG21940.11427363065658377No Hit
GGCAGAGCGTGCCTCTGGAGGAGAGCCTGCCCACCTGCCAGAGGGGCGAC21810.11359653074840895No Hit
GTGTGTGGCCCAGGACCTGGCGGTAGTCAGCCTTGCCCTGGGCGATCAGG21780.11344027692344552No Hit
GTTTCAAACAGCAGCACCACCAGGTCCTTGACAGCTTTGTCGTTCTTGTC21760.11333610770680323No Hit
AGCAGGAACAGCAGCACTGGGTTCCTCTGCTGTCACTTCATCTTCATCAA21610.11255483858198612No Hit
ATCTTCATCAATGCCCAGGCCTAGCTTAATCATGCGGTAGATGCGGTTGG21470.11182565406549015No Hit
GACCGAGACCACCCACTATCCCGAGAACTTCGACCTGAAGGGCCCTCTGC21460.111773569457169No Hit
GCGATAGAGTCCACGAAGTAGTGAAACTTCCTCTTGAAGACATCCAGTGT21390.11140897719892101No Hit
GCACTGGGTTCCTCTGCTGTCACTTCATCTTCATCAATGCCCAGGCCTAG21310.11099230033235188No Hit
ACCAAGGAGGGCCTGGAGCTGCCTGAGGATGAAGAGGAGAAAAAGAAAAT21280.11083604650738846No Hit
AGGCTACATCAGCTACCCGCGGACCGAGACCACCCACTATCCCGAGAACT21270.11078396189906732No Hit
GTGCACAGGGATGCTGGCGTCTGTCCCAATGCCATGCTTCTCCATGAGTG21230.11057562346578276No Hit
ATCTCTAACAGGCTAGTGTCTTCACCCTGCTGCATTGTGACTAGCACCTA21200.11041936964081933No Hit
ACTCTCCACAGTGACATAGTTGCGTTGACAGATGTTGTTGATGTGCACAG21080.10979435434096563No Hit
CCACAGTGACATAGTTGCGTTGACAGATGTTGTTGATGTGCACAGGGATG21080.10979435434096563No Hit
CATTACCCCCATGAGGTCTGCCACTGAGGCCGAATTAGGGGGCGAGGCAT21060.10969018512432337No Hit
AGTCTAGTCTCTGTTACCAAGGAGGGCCTGGAGCTGCCTGAGGATGAAGA20910.10890891599950625No Hit
CTGCCAGGGCATGATCTCCGTGAAGCCTGGAGAGATGACGGTTTTGCCTG20640.10750263157483543No Hit
AGCGAAAGCAGGTCTCGTCACAGTGTGAGCAGTGCAGGCGGCTCGGCTTG20620.10739846235819314No Hit
GAAGAGAGCAGAGCTGTTTCAAACAGCAGCACCACCAGGTCCTTGACAGC20500.10677344705833944No Hit
GCCATGCAGACCGCCGAGCGGCTCTACACACAAGGCTACATCAGCTACCC20350.10599217793352232No Hit
CCATGTTGGCTGTCCAGCCATAGGTGCTAGTCACAATGCAGCAGGGTGAA20320.10583592410855891No Hit
GTAGATGCGGTTGGAGTGAGTCTGGGGATCCTCCAGTGAGAAGCCAGAAG20100.1046900627254938No Hit
GTGTGGCCCAGGACCTGGCGGTAGTCAGCCTTGCCCTGGGCGATCAGGTT20030.10432547046724581No Hit
GTCAGGTAGTCGGGCGGGCTCGTCTGTTTCTCCAGCATCTTTATCTCGCT19990.10411713203396125No Hit
CACTCATGGAGAAGCATGGCATTGGGACAGACGCCAGCATCCCTGTGCAC19970.10401296281731895No Hit
GCCATAGGTGCTAGTCACAATGCAGCAGGGTGAAGACACTAGCCTGTTAG19960.10396087820899783No Hit
CATGGAGAAGCATGGCATTGGGACAGACGCCAGCATCCCTGTGCACATCA19760.102919186042575No Hit
GAGCAGAGCTGTTTCAAACAGCAGCACCACCAGGTCCTTGACAGCTTTGT19680.10250250917600587No Hit
ACACACAAGGCTACATCAGCTACCCGCGGACCGAGACCACCCACTATCCC19620.10219000152607903No Hit
ATATTACATGGTATGCAATTCTGTTCTTTCTTCACATTCATGAAACAAAA19600.10208583230943674No Hit
GCCATGCACCAGGACGATGCCCAGGTTGGTGGGCTTGAGCCGGCGCCCAC19550.10182540926783104No Hit
GCTCTGGAGGTACTTGCAGTCGTAGCTCACCGTGGCGATGAAGTGTCTGG19230.10015870180155452No Hit
GGGTTCCTCTGCTGTCACTTCATCTTCATCAATGCCCAGGCCTAGCTTAA19220.10010661719323337No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)